Bio-oil based biorefinery strategy for the production of succinic acid
1 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P. O. Box 353, No. 1 Zhongguancun North Second Street, Beijing 100190, P.R. China
2 2University of Chinese Academy of Sciences, Beijing 100049, R.P. China
3 Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, DK-2800, Denmark
4 Sino-Danish Center for Education and Research, Niels Jensensvej 2, DK-8000, Aarhus C, Denmark
5 State Key Laboratory of Multiple Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Zhongguancun North Second Street, P. O. Box 353, , Beijing 100190, P.R. China
Biotechnology for Biofuels 2013, 6:74 doi:10.1186/1754-6834-6-74Published: 8 May 2013
Succinic acid is one of the key platform chemicals which can be produced via biotechnology process instead of petrochemical process. Biomass derived bio-oil have been investigated intensively as an alternative of diesel and gasoline fuels. Bio-oil could be fractionized into organic phase and aqueous phase parts. The organic phase bio-oil can be easily upgraded to transport fuel. The aqueous phase bio-oil (AP-bio-oil) is of low value. There is no report for its usage or upgrading via biological methods. In this paper, the use of AP-bio-oil for the production of succinic acid was investigated.
The transgenic E. coli strain could grow in modified M9 medium containing 20 v/v% AP-bio-oil with an increase in OD from 0.25 to 1.09. And 0.38 g/L succinic acid was produced. With the presence of 4 g/L glucose in the medium, succinic acid concentration increased from 1.4 to 2.4 g/L by addition of 20 v/v% AP-bio-oil. When enzymatic hydrolysate of corn stover was used as carbon source, 10.3 g/L succinic acid was produced. The obtained succinic acid concentration increased to 11.5 g/L when 12.5 v/v% AP-bio-oil was added. However, it decreased to 8 g/L when 50 v/v% AP-bio-oil was added. GC-MS analysis revealed that some low molecular carbon compounds in the AP-bio-oil were utilized by E. coli.
The results indicate that AP-bio-oil can be used by E. coli for cell growth and succinic acid production.