Table 2 |
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|
Metabolites differing in relative abundance in cell pellets and culture supernatants of Clostridium cellulolyticum mdh, ldh and ldh mdh mutants compared to wild-type at stationary growth phase |
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|
Compound |
Cell pellets: abundance ratio to wild-type |
Culture supernatants: abundance ratio to wild-type |
||||
|
|
||||||
|
mdh |
ldh |
mdh-ldh |
mdh |
ldh |
mdh-ldh |
|
|
|
||||||
|
Malic acid |
0.32 |
1.48 |
0.28 |
0.84 |
1.44 |
0.57 |
|
2-Hydroxyglutaric acid |
0.28 |
1.42 |
12.43 |
0.35 |
1.72 |
4.94 |
|
Valine |
0.27 |
1.01 |
0.37 |
0.35 |
1.17 |
0.68 |
|
Glucose-1-P |
0.90 |
1.20 |
0.56 |
0.89 |
1.36 |
0.57 |
|
Fructose-6-P |
0.70 |
0.99 |
0.79 |
0.99 |
2.89 |
1.39 |
|
Glucose-6-P |
0.39 |
1.11 |
1.12 |
1.41 |
9.21 |
4.69 |
|
Succinic acid |
0.51 |
0.56 |
1.12 |
0.58 |
0.92 |
0.62 |
|
Fumaric acid |
0.31 |
0.70 |
0.40 |
0.48 |
0.79 |
0.36 |
|
p-Hydroxybenzoic acid |
0.53 |
0.65 |
1.24 |
0.46 |
0.98 |
1.77 |
|
10.13 191 246 376 348 |
0.22 |
0.88 |
0.25 |
0.35 |
1.13 |
0.90 |
|
Citric acid |
0.12 |
0.96 |
6.03 |
ND |
ND |
ND |
|
11.05 191 276 |
0.44 |
1.53 |
0.18 |
ND |
ND |
ND |
|
4-Methyl-2-hydroxypentanoic acid |
ND |
ND |
ND |
0.17 |
0.91 |
2.63 |
|
Pyruvic acid |
0.56 |
0.45 |
0.88 |
0.18 |
2.25 |
0.95 |
|
Galactose |
ND |
ND |
ND |
3.41 |
2.67 |
2.32 |
|
2,3-Butanediol |
ND |
ND |
ND |
0.29 |
1.76 |
1.92 |
|
9.97 331 359 226 |
ND |
ND |
ND |
0.11 |
1.62 |
2.12 |
|
11.07 191 348 320 246 |
ND |
ND |
ND |
0.73 |
1.46 |
0.44 |
|
|
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|
Metabolites were analyzed by gas chromatography-mass spectrometry (GC-MS) as trimethylsilyl (TMS) derivatives, using a sorbitol internal standard [42]. Peaks that could not be assigned to a standard metabolite are identified by their chromatographic retention times (in min) and their characteristic ion peaks (m/z in italic text). ND = not determined (the concentrations of these metabolites were too low to produce reliable estimates of relative abundance). |
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|
Li et al. Biotechnology for Biofuels 2012 5:2 doi:10.1186/1754-6834-5-2 |
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