Research
Combined inactivation of the Clostridium cellulolyticum lactate and malate dehydrogenase genes substantially increases ethanol yield from cellulose and switchgrass fermentations
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* Corresponding author: David E Graham grahamde@ornl.gov
1 Biosciences Division, Oak Ridge National Laboratory, PO Box 2008, MS-6038, Oak Ridge, TN 37831-6038, USA
2 BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
3 Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA
4 Department of Microbiology, University of Tennessee, Knoxville, TN 37996-0845, USA
Biotechnology for Biofuels 2012, 5:2 doi:10.1186/1754-6834-5-2
Published: 4 January 2012Additional files
Additional file 1:
PCR analysis of intron insertions in Clostridium cellulolyticum mdh and ldh genes. This file contains an image of an ethidium bromide-stained agarose gel illustrating PCR products from erythromycin-resistant C. cellulolyticum colonies.
Format: PDF Size: 604KB Download file
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Additional file 2:
Sequences of the mdh and ldh gene disruptions, and the proposed ack and pta disruptions. This file contains diagrams and sequences of the gene disruption constructs created for this project.
Format: PDF Size: 1.2MB Download file
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Additional file 3:
Phylogeny of Clostridial ldh and mdh paralogs. This file contains a phylogenetic tree showing the most likely evolutionary history of mdh and ldh paralogs in Clostridium cellulolyticum and related organisms.
Format: PDF Size: 764KB Download file
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Additional file 4:
Maximum fermentation product concentrations of Clostridium cellulolyticum wild-type and mutant strains grown with different carbon sources. This file contains a table of primary fermentation product concentrations for strains grown on defined medium with glucose, cellobiose, xylose, xylan, pretreated switchgrass or Avicel.
Format: PDF Size: 81KB Download file
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Additional file 5:
Colony PCR screen for intron insertions in the phosphotransacetylase (pta) and acetate kinase (ack) genes. This file contains images of ethidium bromide-stained agarose gels illustrating PCR products from erythromycin-resistant Clostridium cellulolyticum colonies.
Format: PDF Size: 1010KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 6:
Clostridium cellulolyticum plasmids and strains. This file contains a list of plasmid vectors and C. cellulolyticum strains used in this project, along with a list of relevant features or genotypes.
Format: PDF Size: 91KB Download file
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Additional file 7:
Oligonucleotide primers used for targeted mutagenesis. This file contains a table of all oligonucleotides used in this project.
Format: PDF Size: 70KB Download file
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