PCR analysis of the genotype of Synechocystis sp. PCC 6803 mutant strains. (A) lane 1: DNA marker (200 bp DNA Ladder Marker), lane 2: genomic DNA of GQ8 was amplified by primers kus and kdas outside the inserted gene segment, lane 3: plasmid pGQ53 was amplified by primers kvF and kvR located inside slr1609 gene (control), lane 4: genomic DNA of GQ8 was amplified by the same primers as lane 3, lane 5: genomic DNA of wild-type was amplified by the same primers as lane 3 (control). (B) lane1: DNA marker (1 kb DNA Ladder Marker), lane2: genomic DNA of wild type was amplified by primers 0168-2 and 1609NdeI (control), lane3: genomic DNA of GQ3 was amplified by the same primers as lane2, lane4: plasmid pGQ11 was amplified by the same primer as lane2 (control), lane5: genomic DNA of wild-type was amplified by primers 0168-1 and 0168-2(control), lane6: genomic DNA of GQ3 was amplified by the same primers as lane5, lane7: H2O was used as template, and the same primer as lane5 were used in PCR reaction(control).
Gao et al. Biotechnology for Biofuels 2012 5:17 doi:10.1186/1754-6834-5-17