Biotechnology for Biofuels

official impact factor 4.15

Open Access Research

A versatile toolkit for high throughput functional genomics with Trichoderma reesei

André Schuster1,2, Kenneth S Bruno1, James R Collett1, Scott E Baker1, Bernhard Seiboth2, Christian P Kubicek2* and Monika Schmoll2

Author Affiliations

1 Chemical and Biological Process Development, Energy and Environment Directorate, Pacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA, USA

2 Research Area of Gene Technology and Applied Biochemistry, Institute for Chemical Engineering, Vienna University of Technology, Gumpendorfer Strasse 1a/1665, A-1060 Wien, Austria

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Biotechnology for Biofuels 2012, 5:1 doi:10.1186/1754-6834-5-1

Published: 2 January 2012

Additional files

Additional file 1:

Oligonucleotides used for vector construction and screening. This file contains primer sequences, the respective fragment sizes of the amplicons prepared for vector construction along with the size of the amplicon for transformant screenings.

Format: XLS Size: 46KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 2:

Oligonucleotides for high throughput yeast mediated vector construction using the pyr4 marker system.

Format: XLS Size: 3MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Oligonucleotides for high throughput yeast mediated vector construction using the amdS marker system.

Format: XLS Size: 3MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 4:

Oligonucleotides for high throughput yeast mediated vector construction using the hph marker system.

Format: XLS Size: 3MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data