Methodology
Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
Biotechnology for Biofuels 2011, 4:61 doi:10.1186/1754-6834-4-61
Published: 22 December 2011Abstract (provisional)
Here is described a method based on BODIPY staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about three weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.