Figure 6.

Glucose (Glc) yield as a function of enzyme loading for different pretreatment conditions, H₂O₂ loadings, and enzyme hydrolysis times. The enzymes used for the hydrolysis were mixtures of four commercial enzymes as established for each specific pretreatment condition (that is, H₂O₂ loading) at an enzyme loading of 15 mg/g glucan and 48 h hydrolysis (see Table 1). (a) Alkaline hydrogen peroxide (AHP) pretreatment and 24 h enzyme hydrolysis. (b) AHP pretreatment and 48 h digestion. (c) Ammonia fiber expansion (AFEX) pretreatment, 24 h and 48 h enzyme hydrolysis. Biomass loading during the AHP pretreatment was 10%. For AFEX conditions see Methods. All biomass loadings in the enzyme hydrolysis step were 0.2% glucan. Glc yield values indicate yield as a percentage of total measured monomeric Glc content of the biomass. Numbers next to the data points are the Glc yields (y axis values).