Biotechnology for Biofuels

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Open Access Research

Furfural induces reactive oxygen species accumulation and cellular damage in Saccharomyces cerevisiae

Sandra A Allen1, William Clark1, J Michael McCaffery2, Zhen Cai1, Alison Lanctot1, Patricia J Slininger3, Z Lewis Liu3 and Steven W Gorsich1*

Author Affiliations

1 Biology Department, Central Michigan University, Mt Pleasant, MI 48859, USA

2 Integrated Imaging Center, Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA

3 National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA

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Biotechnology for Biofuels 2010, 3:2 doi:10.1186/1754-6834-3-2

Published: 15 January 2010

Additional files

Additional file 1:

Figure S1 - Furfural causes exponentially growing yeast to enter a growth lag phase. Exponentially growing yeast cells in synthetic complete medium were either untreated (circle) or treated with 25 mM (square) or 50 mM furfural (triangle) and allowed to continue to grow at 30°C. At the indicated time points aliquots of cells were removed and cell density measured (A600).

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