Table 2 |
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|
Langmuir and kinetic adsorption parameters of purified cellulases using α-cellulose at n = 1000 rpm |
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|
Cellulase |
Langmuir adsorption parameters* |
Kinetic adsorption parameters† |
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|
Amax [μmol/g] |
KD [μmol/L] |
R² [-] |
Amax [μmol/g] |
kad [s-1] |
R² [-] |
|
|
|
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|
CBH I |
0.155‡ ± 0.003 |
0.433 ± 0.039 |
0.93 |
0.170‡ ± 0.003 |
0.0031 ± 0.0002 |
0.98 |
|
EG I |
0.212 ± 0.010 |
2.146 ± 0.216 |
0.90 |
0.213 ± 0.007 |
0.0019 ± 0.0002 |
0.96 |
|
|
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|
* According to Eqn (1). † According to Eqn (2). ‡ Differences of Amax resulted because different charges of CBH I were used. Errors are given as standard deviations. Amax, the maximum cellulase adsorption at equilibrium; KD, the dissociation constant; kad, the pseudo-first-order adsorption rate constant; CBH, cellobiohydrolase; EG, endoglucanase. |
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|
Jäger et al. Biotechnology for Biofuels 2010 3:18 doi:10.1186/1754-6834-3-18 |
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