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Forage quality and composition measurements as predictors of ethanol yield from maize (Zea mays L.) stover

Aaron J Lorenz1 email, Rob P Anex2 email, Asli Isci2 email, James G Coors1 email, Natalia de Leon1 email and Paul J Weimer3 email

Department of Agronomy, University of Wisconsin, Linden Drive, Madison, WI 53706, USA

Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA

USDA-ARS, US Dairy Forage Research Center, Linden Drive West, Madison, WI 53706, USA

author email corresponding author email

Biotechnology for Biofuels 2009, 2:5doi:10.1186/1754-6834-2-5

Published: 9 March 2009

Abstract

Background

Improvement of biofeedstock quality for cellulosic ethanol production will be facilitated by inexpensive and rapid methods of evaluation, such as those already employed in the field of ruminant nutrition. Our objective was to evaluate whether forage quality and compositional measurements could be used to estimate ethanol yield of maize stover as measured by a simplified pretreatment and simultaneous saccharification and fermentation assay. Twelve maize varieties selected to be diverse for stover digestibility and composition were evaluated.

Results

Variation in ethanol yield was driven by glucan convertibility rather than by glucan content. Convertibility was highly correlated with ruminal digestibility and lignin content. There was no relationship between structural carbohydrate content (glucan and neutral detergent fiber) and ethanol yield. However, when these variables were included in multiple regression equations including convertibility or neutral detergent fiber digestibility, their partial regression coefficients were significant and positive. A regression model including both neutral detergent fiber and its ruminal digestibility explained 95% of the variation in ethanol yield.

Conclusion

Forage quality and composition measurements may be used to predict cellulosic ethanol yield to guide biofeedstock improvement through agronomic research and plant breeding.


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