Parallel metatranscriptome analyses of host and symbiont gene expression in the gut of the termite Reticulitermes flavipes

doi:10.1186/1754-6834-2-25

Biotechnology for Biofuels

Volume 2

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Wheeler MM
Zhou X
Coy MR
Boucias DG
Scharf ME

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Parallel metatranscriptome analyses of host and symbiont gene expression in the gut of the termite Reticulitermes flavipes

Aurélien Tartar¹^,2 , Marsha M Wheeler¹^,3 , Xuguo Zhou¹^,4 , Monique R Coy¹ , Drion G Boucias¹ and Michael E Scharf¹

¹Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA

²Division of Math, Science and Technology, Nova Southeastern University, Fort Lauderdale, FL, USA

³Current address : Department of Entomology, University of Illinois, Champaign-Urbana, IL, USA

⁴Current address : Department of Entomology, University of Kentucky, Lexington, KY, USA

author email corresponding author email

Biotechnology for Biofuels 2009, 2:25doi:10.1186/1754-6834-2-25


Published:	15 October 2009

Additional files

Additional file 1:

Table S1 - Carbohydrate active genes, gut (host) library. Summary of glycoside hydrolase (GH), glycosyl transferase (GT), carbohydrate esterase (CE), carbohydrate binding modules (CBM) and other miscellaneous (Misc.) carbohydrate active domain protein coding genes identified from the termite gut (host) library sequencing. Accession Numbers are provided in Additional file 3.

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Additional file 2:

Table S2 - Carbohydrate active genes, SYMBIONT library. Summary of glycoside hydrolase (GH), glycosyl transferase (GT), carbohydrate esterase (CE), carbohydrate binding modules (CBM) and other miscellaneous (Misc.) carbohydrate active domain protein coding genes identified from the symbiont library sequencing. Accession Numbers are provided in Additional file 4.

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Additional file 3:

Table S3. Genbank accession numbers for carbohydrate active enzymes, gut (host) library.

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Additional file 4:

Table S4. Genbank accession numbers for carbohydrate active enzymes, SYMBIONT library.

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Additional file 5:

Table S5 - Dockerin, Fe-hydrogenase, ferredoxin oxidoreductase and nitroreductase genes, SYMBIONT library. Summary of dockerin, Fe-hydrogenase, ferredoxin oxidoreductase and nitroreductase genes identified from the symbiont library sequencing. Accession Numbers are provided in Additional file 10.

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Additional file 6:

Table S6 - Candidate lignase, detoxification and antioxidant genes, gut (host) library. Summary of candidate lignin degradation, detoxification and antioxidant enzyme coding genes identified from the termite gut (host) library sequencing. Accession Numbers are provided in Additional file 7.

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Additional file 7:

Table S7. Genbank accession Nos. for candidate lignase, detoxification and antioxidant genes, gut (HOST) library.

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Additional file 8:

Figure S1. Deduced amino acid alignment of the R. flavipes laccase contig obtained in the present study (Contig 659; indicated by arrows) with homologous insect and fungal laccases. The fungal laccases shown play known roles in lignin degradation. Shaded amino acids are those that match the R. flavipes sequence; insect sequences are above the arrows and fungal sequences are below. The ESTs assembling into the R. flavipes contig are as follows: FL639514, FL640712, FL635040, FL635071, FL635132, and FL635524. Sequence accession numbers for all homologs are shown in parentheses: Ms (Manduca sexta), Tribolium (T. castaneum), Ag (Anopheles gambiae), Termitomyces (Termitomyces sp. NS/Mg.), C. cinera (Coprinus cinereus), A. bisporus (Agaricus bisporus).

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Additional file 9:

Table S8. PCR primer sequences used for validating laccase and catalase gene expression relative to the control gene β-actin.

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Additional file 10:

Table S9. Genbank accession Nos. for Dockerin, Fe-hydrogenase, ferredoxin oxidoreductase and nitroreductase genes, SYMBIONT library.

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