Table 4 |
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|
Effect of Conditions on Filter Paper Assaya |
|||||
|
Filer paper mg |
25 |
50 |
50 |
100 |
|
|
Enzyme ml |
0.5 |
0.5 |
1.0 |
0.5 |
|
|
Enzyme protein mg |
0.7 |
0.7 |
1.4 |
0.7 |
|
|
|
|||||
|
Activity mg glucose |
30 min |
2.29 |
2.43 |
3.52 |
5.18 |
|
60 min |
3.30 |
3.68 |
4.40 |
5.53 |
|
|
120 min |
4.60 |
5.30 |
6.75 |
7.16 |
|
|
|
|||||
|
Activity per h |
30 min |
4.58 |
4.86 |
7.04 |
10.36 |
|
60 min |
3.30 |
3.68 |
4.40 |
5.53 |
|
|
120 min |
2.30 |
2.65 |
3.38 |
3.58 |
|
|
|
|||||
|
Units/ml |
30 min |
0.93 |
1.23 |
1.19 |
4.35 |
|
60 min |
0.76 |
1.23 |
1.23 |
3.52 |
|
|
120 min |
0.80 |
1.42 |
1.32 |
3.08 |
|
|
|
|||||
|
Units/mg protein |
30 min |
0.66 |
0.88 |
0.85 |
3.11 |
|
60 min |
0.54 |
0.88 |
0.88 |
2.51 |
|
|
120 min |
0.57 |
1.01 |
0.94 |
2.61 |
|
|
|
|||||
|
a0.5 or 1.0 ml of enzyme (culture filtrate of QM9414 grown on cellulose) + 1 ml of pH 4.8 buffer + Strip of Whatman No. 1 filter paper. Units = μmol of glucose per min based on the dilution to give 2.0 mg glucose. |
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|
Eveleigh et al. Biotechnology for Biofuels 2009 2:21 doi:10.1186/1754-6834-2-21 |
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