Table 2

Endo β Glucanase (Cx) Assaya
Reference
 Enzyme, ml
 CMC, ml
 CMC, % in assay
 Time, min
 1 Cx units/ml Glucose mg/ml
 1 Cx units/ml Glucose total mg
 International units
Cx units
Ab [6]
 1.0
 9.0
 0.5
 60
 0.4
 4.0
 0.37
Bc [7]
 0.5
 4.5
 0.5
 60
 0.4
 2.0
 0.37
Cd [8]
 0.5
 0.5
 0.5
 30
 0.5
 0.5
 0.185

aSubstrate = carboxymethyl cellulose (CMC), degree of substitution 0.5. Temperature = 50 C. Product = reducing sugar as glucose. Buffer = 0.05 M citrate pH 4.8. Calculations: 1 IU = 1 μmol of glucose/min (0.18 mg/min). 1 mg glucose/0.18 × 60 = 0.0925 units (1 h) or 1 mg glucose/0.18 × 30 = 0.185 units (0.5 h).

b4.0 mg glucose × 0.0925/1.0 ml enzyme = 0.37 IU/Cx unit.

c2.0 mg glucose × 0.0925/0.5 ml enzyme = 0.37 IU/Cx unit.

d0.5 mg glucose × 0.185/0.5 ml enzyme = 0.185 IU/Cx unit.

Eveleigh et al. Biotechnology for Biofuels 2009 2:21   doi:10.1186/1754-6834-2-21

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