Table 1 |
||
|
Cellulase Assays |
||
| Enzyme |
Substrate |
Product measured |
|
|
||
| Cellobiase |
||
| β Glucosidase |
Cellobiose |
Glucose |
| Cellodextrins |
||
| Salicin |
Salingenin |
|
| p-Nitro β glucosidase |
p Nitrophenol |
|
|
|
||
| Endo β 1, 4 glucanase |
||
| Cx |
Carboxymethyl cellulose |
Loss in viscosity |
| CMC'ase |
Amorphous cellulose |
Reducing sugar |
| Walseth |
||
| Sweco |
||
| Cellodextrins |
||
|
|
||
| Exo β 1, 4 Glucanase |
||
| A Glucocellulase |
Amorphous cellulose |
Glucose (A) |
| Walseth |
||
| B Cellobiohydrolase |
Crystalline cellulose |
Cellobiose (B) |
| CBH |
Avicel |
|
| C1 |
Cellodextrins |
|
|
|
||
| Cellulase |
||
| C1 + Cx |
Crystalline cellulose |
Loss in weight |
| Avicellase |
Avicel |
Reducing sugar |
| Hydrocellulase |
Hydrocellulose |
Reduction in optical density (OD) |
| FP'ase |
Filter paper |
|
| Cellodextrins |
||
| Cotton |
||
|
|
||
| Miscellaneous |
||
| Swelling factor |
Cotton |
Uptake of alkali |
| Filter paper cellulase |
Filter paper |
Maceration [1] |
| Thread |
Breaking strength |
|
| Dyed cellulose |
Release of dye |
|
|
|
||
|
CMC = carboxymethyl cellulose; FP = filter paper. |
||
|
Eveleigh et al. Biotechnology for Biofuels 2009 2:21 doi:10.1186/1754-6834-2-21 |
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