Replacement of water. Hydrolysis of filter paper at large laboratory scale with 20% initial solids content (w/w) and an enzyme dosage of 10 FPU per gram dry matter (DM) where 25% of the water was replaced with the inert oil, oleyl alcohol. This corresponds to an increase of biomass to water ratio of 25%. The yield was found to be less than the reference, but not as low as a 25% increase in solids normally results in. Points are averages of two experiments.
Kristensen et al. Biotechnology for Biofuels 2009 2:11 doi:10.1186/1754-6834-2-11