Figure 1.

Effect of substrate concentration on the enzymatic digestibility of eucalyptus treated with ball milling. To prepare a large quantity of the sample, ball-milling (BM) treatment (240-minute milling) was performed using pulverisette 5 as described in materials and methods. Various amounts of substrates were mixed and hydrolyzed for 72 hours with the same volume of enzyme cocktail (4 FPU/g substrate). Yields of hydrolyzed glucose and xylose are expressed as a percentage of the theoretical amount based on the glucan and xylan contents, respectively, in the dry weight of untreated biomass.